Fig. 5.
Fig. 5. The effect of HMA on the pHi and apoptosis of normal peripheral blood cells. / Not shown on the graphs are the pHi and apoptosis control time points in the absence of HMA. These are as follows: (A) Fluorescence ratio imaging microscopy (FRIM), 30 minutes = 6.95 ± 0.02, 1 hour = 7.00 ± 0.015, 3 hours = 7.08 ± 0.02, 5 hours = 7.13 ± 0.02. (B) Flow cytometry, 30 minutes = 7.01 ± 0.03, 1 hour = 6.98 ± 0.04, 3 hours = 7.04 ± 0.04, 5 hours = 7.09 ± 0.05. (C) Annexin-V-FITC, 30 minutes = 1.53 ± 0.09, 1 hour = 1.32 ± 0.15, 3 hours = 1.38 ± 0.04, 5 hours = 2.6 ± 0.07. (D) TUNEL, 30 minutes = 0.89 ± 0.31, 1 hour = 0.96 ± 0.16, 3 hours = 0.60 ± 0.29, 5 hours = 1.8 ± 0.48. Results represent mean ± SEM of 3 experiments.

The effect of HMA on the pHi and apoptosis of normal peripheral blood cells.

Not shown on the graphs are the pHi and apoptosis control time points in the absence of HMA. These are as follows: (A) Fluorescence ratio imaging microscopy (FRIM), 30 minutes = 6.95 ± 0.02, 1 hour = 7.00 ± 0.015, 3 hours = 7.08 ± 0.02, 5 hours = 7.13 ± 0.02. (B) Flow cytometry, 30 minutes = 7.01 ± 0.03, 1 hour = 6.98 ± 0.04, 3 hours = 7.04 ± 0.04, 5 hours = 7.09 ± 0.05. (C) Annexin-V-FITC, 30 minutes = 1.53 ± 0.09, 1 hour = 1.32 ± 0.15, 3 hours = 1.38 ± 0.04, 5 hours = 2.6 ± 0.07. (D) TUNEL, 30 minutes = 0.89 ± 0.31, 1 hour = 0.96 ± 0.16, 3 hours = 0.60 ± 0.29, 5 hours = 1.8 ± 0.48. Results represent mean ± SEM of 3 experiments.

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