Replication and package of rAAV/BDD-hFVIII.

Low molecular weight DNA (Hirt DNA) was isolated from rAAV/DLZ2, DLZ6 and DLZ8 (control) transduced HeLa and HepG2 cells, separated by agarose gel, and probed with BDD-hFVIII cDNA. From right to left: Control Lane, 1-HepG2+ rAAV/DLZ8; 2-HeLa + rAAV/DLZ8; DLZ2: 1-HeLa + rAAV/DLZ2; 2-HepG2 + rAAV/DLZ2; DLZ6: 1-HeLa + rAAV/DLZ6; 2-HepG2 + rAAV/DLZ6; and uncoated rAAV/DLZ6 virion DNA.