IgE-Ag–mediated interleukin 10 (A), interleukin 6 (B), and JunD (C) mRNA accumulation in bone marrow mast cells from PKCβ-deficient and wild-type mice.

RNA isolated from IgE-Ag–stimulated mast cells was reverse transcribed with specific primers and then amplified. Polymerase chain reaction products were resolved on 2% agarose gel containing 0.008% ethidium bromide.