GCP-2–induced downmodulation of CXCR1 and CXCR2 expression.

CXCR1- or CXCR2-transfected HEK 293 cells incubated with 3000 and 1000 ng/mL GCP-2, respectively, for 2 hours at 37°C. The cells were washed and stained with anti-CXCR1 or anti-CXCR2 specific antibodies, and subjected to fluorescence-activated cell sorting (FACS) analysis as described in “Materials and methods.” (A) CXCR1-expressing cells. (B) CXCR2-expressing cells. Counts indicate relative cell number; baseline, cells stained with cell sorter buffer (CSB) instead of antibodies to CXCR1 or CXCR2. A representative experiment of 4 to 5 performed is shown.