Increases in EPCR mRNA levels in rat lungs with infusion of thrombin.

Bovine thrombin (5 U/kg infused per hour) and hirudin (100 U/kg infused per hour) were infused into the femoral vein of rats, and the lungs and hearts were removed 3 hours later for the extraction of total RNA. (A) Northern blot analysis. (B) Below the Northern blot is the quantitation of the change based on normalization of the signal of EPCR mRNA to that of CHO-B mRNA on the PhosphorImager.