Concomitant detection of Tax and CD4 antigens in peripheral blood mononuclear cells (PBMCs) isolated from a patient infected with human T-cell leukemia virus type I (TAR).

PBMCs were isolated from blood either before or after cultivation for 12 hours. Either heparin or EDTA was used as anticoagulant. The Tax protein was detected with the Lt-4 monoclonal antibody (D, E, F), and an irrelevant monoclonal antibody was used as a control isotype (A, B, C). One representative experiment of 3 is shown.