C5 induces mitochondrial swelling by induction of the MPT pore.

Large-amplitude mitochondrial swelling was determined spectroscopically by monitoring the loss of absorbance at 540 nm, as described in “Materials and methods.” Mitochondria (0.5 mg) were treated with (A) 200 μmol/L DEPC as a positive control to induce mitochondrial swelling or (B) C5 (150 μg) in the absence or presence of MPT pore inhibitor CsA (10 μmol/L).