Immunohistochemical and Western Blot detection of TβR-II protein.

(A) Example of a thymic lymphoma with wild-type TβR-II (A) repeat and detectable mRNA by RT-PCR. All tumor cells show strong immunohistochemical staining for TβR-II. (B) Example of a thymic lymphoma harboring an (A) repeat ID mutation, and showing no detectable mRNA by RT-PCR, demonstrates the absence of immunohistochemical staining for protein. (C) Nonmalignant tissue from the small intestine of the animal from panel B shows strong immunohistochemical staining for TβR-II. (D) Representative Western analysis of 2 murine lymphomas. Lane 1, lymphoma harboring TβR-II (A) repeat ID mutation; lane 2, lymphoma showing wild-type TβR-II (A) repeat. Top panel demonstrates absence of TβR-II protein in lane 1. Bottom panel is the same blot re-probed with an antibody to TβR-I as control.