American Society of Hematology

Fig. 4.

$Fig. 4. Detection of SF-MDR provirus in cells obtained from chimeric NOD/SCID mice. / (A) PCR analysis of the mononuclear cell (MNC) fraction and the granulocyte (polymorphonuclear; PMN) fractions of BM recovered from 11 mice (NOD/SCID set 7) engrafted with mock-transduced (1 and 2) orMDR1-transduced (3-11) CD34+ PBPC. (B) Detection of the SF-MDR proviral genome in individual colonies derived from 2 chimeric mice (NOD/SCID sets 2 and 8) engrafted with Transwell-transduced CD34+ PBPC. (C) Detection of the SF-MDR proviral genome in individual colonies derived from 2 chimeric mice (NOD/SCID set 10) engrafted with supernatant-transduced CD34+ PBPC. M denotes molecular-weight size marker; Nc, no-template PCR control; and Pc, SF-MDR producer cells diluted 10−5-fold in provirus-negative HL-60 cells.$

Detection of SF-MDR provirus in cells obtained from chimeric NOD/SCID mice.

(A) PCR analysis of the mononuclear cell (MNC) fraction and the granulocyte (polymorphonuclear; PMN) fractions of BM recovered from 11 mice (NOD/SCID set 7) engrafted with mock-transduced (1 and 2) orMDR1-transduced (3-11) CD34+ PBPC. (B) Detection of the SF-MDR proviral genome in individual colonies derived from 2 chimeric mice (NOD/SCID sets 2 and 8) engrafted with Transwell-transduced CD34+ PBPC. (C) Detection of the SF-MDR proviral genome in individual colonies derived from 2 chimeric mice (NOD/SCID set 10) engrafted with supernatant-transduced CD34+ PBPC. M denotes molecular-weight size marker; Nc, no-template PCR control; and Pc, SF-MDR producer cells diluted 10⁻⁵-fold in provirus-negative HL-60 cells.

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