Expression of bcl-XL protein is elevated in fibroblast growth factor receptor 3 (FGFR3)-overexpressing cells.
Total cell lysates were prepared at varying time points from FGFR3-expressing clones or from control cells grown in the absence of interleukin-6 (IL-6) over a 48-hour period. The lysates were resolved via SDS-PAGE, and the membrane was probed with an anti–bcl-xS/L antibody. Membranes were reprobed with ERK as a protein-loading control. (A) bcl-xL is overexpressed at baseline in cells expressing mutant FGFR3 (B9-TD#14) and remains high after IL-6 withdrawal (top panel). (B) bcl-xL is also overexpressed at baseline in cells expressing high levels of wild-type FGFR3 (B9-WT#3 and B9-WT#6) and remains high after IL-6 withdrawal (first and third panel). With the addition of fibroblast growth factor ligand, down-regulation of bcl-xL is noted.
