Fig. 8.
![Fig. 8. Stat6 decoy specifically blocked Stat6 DNA binding activity. / Control (lanes a, b, c, d) and Stat6 decoy ODN–treated (lanes e, f) or scrambled decoy ODN–treated (lanes g, h) D10 cells were incubated with medium (−) or 100 nmol/L IL-4 (+) for 10 minutes at 37°C. Nuclear extracts were obtained, and 5 μg of protein were incubated in the absence of antibody (lanes a, b, e through h), α-Stat6 (lanes c), or normal rabbit serum (nrs; lane d) and then with a [32P]-labeled Stat6 oligonucleotide probe corresponding to the Cε gene promoter. Arrows indicate migrational location of each nonsupershifted Stat6-DNA complex or free probe.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/4/10.1182_blood.v95.4.1249.004k39_1249_1257/6/bloo00439008w.jpeg?Expires=1722716138&Signature=LMKxoAHJbLqY2d6B07u8~rXD~UiKN1anV8bvsJUIA6yFka7QML1H1ATI78PicZHMsY5XHmYuB6sgWq~u0SPzc1Fc4IiyiqNkhVLN48vJ8ckDsIwHPMe~fYGylYsKx9rISdSeV8OYBbC1IqVuxjglYeFAPesAuxWxyRbpoUOYuKCaG~TPbaYX8ywj1cVusxmN6tVTJ~SJxwlzkXR3gffXHkuW-tgtp9qXTJ17kB0tacvkjW8sZM1GTJ1knu8niWD3i3P5ubzYbRWVeEhlbIVlFHrPh6Na7jj7kSxJS2BvduLHZCpmdauLTnyl-gBvVZefgchee~3od~5u~XO1wOZWYQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stat6 decoy specifically blocked Stat6 DNA binding activity.
Control (lanes a, b, c, d) and Stat6 decoy ODN–treated (lanes e, f) or scrambled decoy ODN–treated (lanes g, h) D10 cells were incubated with medium (−) or 100 nmol/L IL-4 (+) for 10 minutes at 37°C. Nuclear extracts were obtained, and 5 μg of protein were incubated in the absence of antibody (lanes a, b, e through h), α-Stat6 (lanes c), or normal rabbit serum (nrs; lane d) and then with a [32P]-labeled Stat6 oligonucleotide probe corresponding to the Cε gene promoter. Arrows indicate migrational location of each nonsupershifted Stat6-DNA complex or free probe.
