Fig. 5.
![Fig. 5. Stat6 decoy does not alter mRNA expression of IL-4 receptors. / Freshly isolated mRNA was obtained from control (lanes a, b, g), Stat6 decoy (lane c), scramble ODN-transfected D10 cells (lane d), or anti–IL-4 antibody treated (lane f) or anti–IL-3 antibody treated (lane e) cells in the absence (lanes a, e, f) or presence of IL-4 (lanes b through d). RNA was then hybridized with [33P]-labeled RNA probes corresponding to transcripts for individual murine γc-receptors (mCR-1) according to PharMingen protocol (see “Materials and methods”). The Rnase-protected fragments were separated on 5% PAGE, dried, and exposed to x-ray film.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/4/10.1182_blood.v95.4.1249.004k39_1249_1257/6/bloo00439005w.jpeg?Expires=1722715212&Signature=X9QLoCjvyBLeMZ0GEd0KiwVzg9zsojV71w6KUm6KF7EYg5j1x2tA7vWfHvt7oZRXgWtiyq2YMk8J1JYzJaAl26bWmCmYDHt2LEN2AR3rcUKYQuCiyxxiNKvQNJShI5GK58DIsLmSMG4-9~UOKt2sAzsHbJRVgbKi1navkL1~wMu8cB60n247GygKNsR7kxVRYOj27Z8LPCJzWLEewQ8ZlLUm4~XHo3Sm13N2wz7Jh9qnBbXmxka5mDKQb0KGatzQxQLMZimp8vI~QTRCS1MWbc2lt2cEGwFB9k5yn7af3OxFUtZ~d00j4ICYE2rfXZd5QA9a2nCDkNhx2qTLnVylVg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stat6 decoy does not alter mRNA expression of IL-4 receptors.
Freshly isolated mRNA was obtained from control (lanes a, b, g), Stat6 decoy (lane c), scramble ODN-transfected D10 cells (lane d), or anti–IL-4 antibody treated (lane f) or anti–IL-3 antibody treated (lane e) cells in the absence (lanes a, e, f) or presence of IL-4 (lanes b through d). RNA was then hybridized with [33P]-labeled RNA probes corresponding to transcripts for individual murine γc-receptors (mCR-1) according to PharMingen protocol (see “Materials and methods”). The Rnase-protected fragments were separated on 5% PAGE, dried, and exposed to x-ray film.
