Fig. 2.
![Fig. 2. Stat6 decoy inhibits mRNA expression of IL-4 but not other cytokines. / RNA for RPA analysis was obtained from untreated control samples (lanes a, b, g), Stat6 decoy (lane c), scrambled (lane d) ODN-transfected D10 cells, or cells pretreated with anti–IL-3 or anti–IL-4 antibodies (lanes e and f, respectively). Cells were stimulated with IL-4 (lanes b through d). RNA was isolated and hybridized with [33P]-labeled RNA probes corresponding to transcripts for murine cytokines (mCK1). The RNase-protected fragments were separated on 5% PAGE, dried, and exposed to x-ray. Densitometric analysis of IL-4 RNA message compared with L32/GAPDH indicated a greater than 90% reduction in Stat6 decoy–treated samples.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/4/10.1182_blood.v95.4.1249.004k39_1249_1257/6/bloo00439002w.jpeg?Expires=1722715114&Signature=MrECWB-lAL0cukC8LbgwD9W7LrIR1ZUDJie2nSc4GyKGX5jwfV3kwr3Bvef5JVvWASavnpqSjzg2bxEekoYDP~dmVDza~KsABcaf1du3uTLdMiy3LUsCgC36hHDiW4MNCSVo981s6SWPFViOcFy5NTug32kDZNwUXPaOtSHExWQO08Kdv3Ap9kDUO-KOp1qxylNo3TbnOlIjnV9apRqGJMRgAb46~CdE8oOjNjCV~4ZeaTc917kACTYFKdK8Xepk-1cPsft9SU9LbdjqDbJuQsZJ3o8tC1-FtyVbsLzJdLQ-oitCBihxsnF7x2PMiSEjcl7wKGvoaa8MsmkRry9qkw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stat6 decoy inhibits mRNA expression of IL-4 but not other cytokines.
RNA for RPA analysis was obtained from untreated control samples (lanes a, b, g), Stat6 decoy (lane c), scrambled (lane d) ODN-transfected D10 cells, or cells pretreated with anti–IL-3 or anti–IL-4 antibodies (lanes e and f, respectively). Cells were stimulated with IL-4 (lanes b through d). RNA was isolated and hybridized with [33P]-labeled RNA probes corresponding to transcripts for murine cytokines (mCK1). The RNase-protected fragments were separated on 5% PAGE, dried, and exposed to x-ray. Densitometric analysis of IL-4 RNA message compared with L32/GAPDH indicated a greater than 90% reduction in Stat6 decoy–treated samples.
