TAMRA-labeled (ODN) uptake in D10 cells.

Phase-contrast (left panel) and fluorescence (right panel) confocal photomicrographs of D10 cells that were transfected with TAMRA-labeled double-stranded ODN (2 μmol/L) for 6 hours with the use of cationic liposomes. Cells were then stimulated without (A) or with (B) 100 nmol/L IL-4 at 37°C for 10 minutes. Cells were fixed with 5% paraformaldehyde and co-loaded with DAPI and examined by laser scanning confocal microscope.