Effect of CD148 immunodepletion on CD100-associated PTP activity in XG-1 cells.

CD148 immunodepletions with a mixture (1:1) of 2 anti-CD148 mAbs (143-41 and A3) and CD101 immunodepletions (negative control depletions) were performed on cell lysates. Depleted and undepleted lysates were immunoprecipitated with either BD16 (anti-CD100), BB27 (anti-CD101), or a mixture (1:1) of the 2 anti-CD148 mAbs, and the immunoprecipitates were then assayed for PTP activity. All experimental procedures are described in “Materials and Methods.”