Fig. 6.
Fig. 6. LacZ expression in erythroid and megakaryocytic lineages driven by the 4 × GATA promoter. / Hematopoietic cells were generated from ES cells by the addition of TPO (A, E, I), EPO (B, F, J for primitive embryonic erythrocytes and C, G, K for definitive adult erythrocytes), and M-CSF (D, H, L) for megakaryocytes, erythroid cells, and macrophages, respectively. The wild-type (−598/+33) (A-D), the 4 × GATA (E-H), and the 4 × ETS promoters (I-L) were transfected into ES cells; differentiation induction was carried out; and the cells were stained with X-gal. Double staining with AChE was performed to confirmLacZ expression in megakaryocytes (A, E, I). The 4 × GATA promoter was active in megakaryocytes (E), primitive embryonic erythrocytes (F), and definitive adult erythrocytes (G), but was inactive in macrophages (H).

LacZ expression in erythroid and megakaryocytic lineages driven by the 4 × GATA promoter.

Hematopoietic cells were generated from ES cells by the addition of TPO (A, E, I), EPO (B, F, J for primitive embryonic erythrocytes and C, G, K for definitive adult erythrocytes), and M-CSF (D, H, L) for megakaryocytes, erythroid cells, and macrophages, respectively. The wild-type (−598/+33) (A-D), the 4 × GATA (E-H), and the 4 × ETS promoters (I-L) were transfected into ES cells; differentiation induction was carried out; and the cells were stained with X-gal. Double staining with AChE was performed to confirmLacZ expression in megakaryocytes (A, E, I). The 4 × GATA promoter was active in megakaryocytes (E), primitive embryonic erythrocytes (F), and definitive adult erythrocytes (G), but was inactive in macrophages (H).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal