Fig. 4.
Fig. 4. Effect of deletion and mutation of the GATA and ETS binding sites on GPIIb promoter activity in megakaryocytes. / Wild-type promoter, promoters with mutations in the GATA (GATAm) or Ets binding site (ETSm), and promoters with deletions of the proximal GATA and Ets binding sites were analyzed. The percentages ofLacZ-positive cells per AChE-positive cells were calculated on day 8 of differentiation induction in the presence of TPO. The results are shown as the relative percentage of LacZexpression compared with the value for the −598/+33 wild-type promoter. The data are shown as the mean ± standard deviation. GATAm and ETSm show significant differences from the wild type (P < .05 by t test). Each experiment was performed 2 or 3 times, and representative data are shown.

Effect of deletion and mutation of the GATA and ETS binding sites on GPIIb promoter activity in megakaryocytes.

Wild-type promoter, promoters with mutations in the GATA (GATAm) or Ets binding site (ETSm), and promoters with deletions of the proximal GATA and Ets binding sites were analyzed. The percentages ofLacZ-positive cells per AChE-positive cells were calculated on day 8 of differentiation induction in the presence of TPO. The results are shown as the relative percentage of LacZexpression compared with the value for the −598/+33 wild-type promoter. The data are shown as the mean ± standard deviation. GATAm and ETSm show significant differences from the wild type (P < .05 by t test). Each experiment was performed 2 or 3 times, and representative data are shown.

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