FACs analysis of transgene expression in bone marrow c-kit⁺Sca-1⁺ stem/progenitor cells 8 months after transplantation.

Bone marrow from 4 long bones of each mouse was recovered, Sca-1 selected, and analyzed by flow cytometry as described in “Materials and Methods.” (left) c-kit versus Sca-1 staining of live-gated cells. (boxes) Regions used to define c-kit⁺Sca-1⁺ cells. (right) Histograms of NGFR expression on live-gated c-kit⁺Sca-1⁺ cells. (markers) Proportion of NGFR⁺ cells. Two age-matched control mice (BA.1 and B6SJL) and 3 mice transplanted with bone marrow cells transduced with the 1171 vector are shown.