Southern blot analysis of integration pattern and proviral copy number in splenocytes of mice with long-term multilineage transgene expression.

DNA was isolated from splenocytes of mice whose transgene expression is shown in Table 3 and was digested with Hind III (A, lanes 1-4) or Hind III and Bcl I (B,C, lanes 1-4). (A,B) Southern blot was probed with a 1.9-kb Hind III/Hind II vector fragment encoding the NGFR and pol/AS cDNA. (C) The same blot was reprobed with a genomic DNA fragment from the interferon-γ gene. The blot was exposed to autoradiographic film for 4 days in all mice except mouse 211, which was exposed for 8 days. (D) Schematic diagram of the retroviral vector and NGFR pol/AS DNA probe used in A and B.