Fig. 3.
Fig. 3. Sequence-specific cleavage ofBCR-ABLp210 substrate by M1-p210-GS. / Autoradiograph of cleavage products generated by M1GS constructs.BCR-ABLp210 substrate was incubated either alone (lane 1), with M1-p190-GS (lane 2), with M1-p210-GS (lane 3), or with M1-TC-GS (lane 4). Schematic representation of specific complexes and products formed by M1-p210-GS and substrates (lane 3) are indicated on the left. Total digestion ofBCR-ABLp210 substrate can be obtained by M1-p210-GS if the time of incubation is longer (data not shown).

Sequence-specific cleavage ofBCR-ABLp210 substrate by M1-p210-GS.

Autoradiograph of cleavage products generated by M1GS constructs.BCR-ABLp210 substrate was incubated either alone (lane 1), with M1-p190-GS (lane 2), with M1-p210-GS (lane 3), or with M1-TC-GS (lane 4). Schematic representation of specific complexes and products formed by M1-p210-GS and substrates (lane 3) are indicated on the left. Total digestion ofBCR-ABLp210 substrate can be obtained by M1-p210-GS if the time of incubation is longer (data not shown).

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