Fig. 5.
Effect of temperature on proliferation of CD40-activated RPMI 8226 and HS Sultan MM cell lines.
RPMI 8226 (A, B) and HS Sultan (C, D) cells (0.7 × 106 initiating cells/well) were cultured in media (○) and with formalin-fixed NIH3T3/CD40LT (•), NIH3T3/wt (□), or NIH3T3/vt (▪) for up to 144 hours. Tumor cells were cultured at 37°C (A, C) or 30°C (B, D), and DNA synthesis was assessed at 0, 2, 8, 16, 24, 36, 48, 72, 96, 120, and 144 hours using3H-TdR incorporation. Experiments were performed in triplicate, and results are expressed as mean ± SEM.
