Glucose-dependent respiratory burst activity in PMNs from PT patients and healthy volunteers.

Superoxide anion production was determined as SOD-inhibitable cytochrome c reduction. PMNs were isolated from venous blood of healthy volunteers (n = 7) and polytrauma patients between the second and the fifth posttraumatic day (n = 6) and incubated in PBS with or without 11 mmol/L glucose. Cells were stimulated with 100 ng/mL PMA in the presence and absence of SOD. Cytochrome c reduction was measured photometrically (550 nm) every 2 minutes for up to 1 hour, and maximum slope was used to calculate superoxide anion production per minute. The superoxide anion production in the presence of glucose minus that in its absence was defined as glucose dependent. Data shown represent the median ± standard deviation.