Influence of glucose breakdown on superoxide anion production in PMNs.

Superoxide anion production was determined as SOD-inhibitable cytochrome c reduction. PMNs were isolated from venous blood of healthy volunteers (n = 7) incubated in PBS with or without 11 mmol/L glucose (left 2 bars) and 500 μmol/L DHEA (right 2 bars). Cells were stimulated with 100 ng/mL PMA in the presence and absence of SOD. Cytochrome c reduction was measured photometrically (550 nm) every 2 minutes for up to 1 hour, and maximum slope was used to calculate superoxide anion production per minute. Data shown represent the median ± standard deviation.