Lactate production of PMNs from PT patients and healthy volunteers.

PMNs were isolated from venous blood of healthy volunteers (n = 5) and PT patients (n = 5) between the second and fifth posttraumatic day. Cells were cultured in PBS with and without 11 mmol/L glucose. Lactate in the medium was measured every hour for up to 4 hours. Glucose-dependent lactate production is determined by the difference between lactate production in the presence and the absence of glucose. Data shown represent the median ± standard deviation.