Concentration-dependent inhibition of EPO-induced erythroid differentiation of CD34⁺ cells by PKC- ribozyme.

CD34⁺ cells were incubated at 30 000 cells/well in flat-bottomed, 96-well plates with different concentrations of PKC-α ribozyme for 24 hours. The cells were then stimulated with EPO (5 U/mL) and SCF (50 ng/mL) and cultured for 7 days before they were stained with the indicated mAbs. One representative of 4 separate experiments is shown.