Fig. 6.
![Fig. 6. Anti–SNAP-23 antibody inhibits [3H]5-HT release. / (A) The permeabilized platelet exocytosis was performed in the presence of increasing amounts of Fab23 (0-0.16 mg/mL). After 5 minutes of Ca++ stimulation (10 μmol/L), [3H]5-HT release was measured (n = 3). (B) Secretion triggered by 10 μmol/L Ca++ was analyzed in the presence of Fab23 (0.12 mg/mL), Fab23 (0.12 mg/mL) preincubated with His6–SNAP-23 (0.45 mg/mL), His6–SNAP-23 (0.45 mg/mL), human SNAP-23 C-terminal peptide (0.15 mg/mL), and rabbit IgG purified from preimmune sera (0.15 mg/mL) (n = 6).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/3/10.1182_blood.v95.3.921.003k17_921_929/6/bloo00317006x.jpeg?Expires=1722780372&Signature=nlLuOnXyymjKI6VwgtyCjdvMAPhZmRfQTqsh6NiOPtqwSixUTX~bOdeUqSKP~6vZ0aiNQrsVn89AFl8AevZPw3pKMkDR-cjZNVX~u2jQXYLGYuAwPBbhL96Ee4ZD5Kn5-0kUQkqf1nKCIwaS2tHJJvjxFsVUR3rfY7GNyHp66dHabqmZEjtP6h~E3GvwhJFbdusdiW5qZc6Fc56YxVU5XXwzA-jBecb9gcmZX8Gs~pC4~vA657W85fCs5EuPH07yP-zZtPVahIAQHU6BokX5jaw0aN~OVcCV~6TrGFN3L63ToaanjZrBMixpTw24WEammwHMJM1WmlshqQk2NXgs4w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Anti–SNAP-23 antibody inhibits [3H]5-HT release.
(A) The permeabilized platelet exocytosis was performed in the presence of increasing amounts of Fab23 (0-0.16 mg/mL). After 5 minutes of Ca++ stimulation (10 μmol/L), [3H]5-HT release was measured (n = 3). (B) Secretion triggered by 10 μmol/L Ca++ was analyzed in the presence of Fab23 (0.12 mg/mL), Fab23 (0.12 mg/mL) preincubated with His6–SNAP-23 (0.45 mg/mL), His6–SNAP-23 (0.45 mg/mL), human SNAP-23 C-terminal peptide (0.15 mg/mL), and rabbit IgG purified from preimmune sera (0.15 mg/mL) (n = 6).
