Fig. 4.
![Fig. 4. Streptolysin O permeabilization of platelets. / (A) Increasing amounts of SLO (0-1.2 U/mL final concentration) were added to the assay buffer containing 108 platelets. Platelets were incubated at 25°C or 37°C for 10 minutes, chilled on ice for 30 minutes, and further warmed to 25°C or 37°C for 10 minutes. The platelets were sedimented and the supernatants were collected. The activity of lactate dehydrogenase (LDH) and hexosaminidase and the amounts of [3H]5-HT and PDGF in the supernatant were measured (see “Materials and Methods”) and compared with the total activity of Triton X-100–solubilized platelets (n = 4). (B) The time line represents the standard reaction scheme used in the permeabilized platelet exocytosis assay.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/3/10.1182_blood.v95.3.921.003k17_921_929/6/bloo00317004x.jpeg?Expires=1724258696&Signature=RCTMpCSOtJ5hXYPiZnhXt8rDmOod4hC9QXWgR0HJ0LoRUMsWBKCVxjU~yw8nuQIj08AX1mXK5ToMA6iHg~XY~WYzQKmrpYo5q91EHnvXgbUa7VOiH6LUdCaUVMKu1lDKcknDSwWzgjbBIc1YJYI3gdw~a7miqu8yjjzE~ociXuv12WM7aAIARdtUO6WQCVV5NKkg0PY4kf8KRRfIQNuXidkzUdwMNmH7FyIL2in2WH-xeuhu0Yr-E5qhh9pl152GJrlcADkfVGHXs1aVavt6SS1ehaT7yXQuSny8f-LAwhtvCwkNG-AZjW3R-DxYOLd-W1Lh135jUzHMWMLpC~mx4w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Streptolysin O permeabilization of platelets.
(A) Increasing amounts of SLO (0-1.2 U/mL final concentration) were added to the assay buffer containing 108 platelets. Platelets were incubated at 25°C or 37°C for 10 minutes, chilled on ice for 30 minutes, and further warmed to 25°C or 37°C for 10 minutes. The platelets were sedimented and the supernatants were collected. The activity of lactate dehydrogenase (LDH) and hexosaminidase and the amounts of [3H]5-HT and PDGF in the supernatant were measured (see “Materials and Methods”) and compared with the total activity of Triton X-100–solubilized platelets (n = 4). (B) The time line represents the standard reaction scheme used in the permeabilized platelet exocytosis assay.
