Fig. 1.
Myeloid dendritic cell culture.
Low-density cells (ρ = 1.077 g/cm3) were isolated from WT and PU.1−/−E16.5 embryos. The cells were cultured (2 × 105 cells/well) in media containing GM-CSF and IL-4 for 3 days. Cells were then fixed and stained for the DC markers CD11c or DEC-205. (A) Photomicrograph of CD11c stained DC from WT or PU.1−/−embryos. (B) Stained cells were counted, and the results were tabulated as DC per 105low-density cells plated in the original culture. Results are given in mean ± SEM (standard error of the mean) per number of embryos tested (n).
