Chelating agents inhibit Loxosceles venom-induced cleavage of glycophorins and induction of C susceptibility.

E were incubated with EDTA (10 mmol/L), 1,10-phenanthroline (5 mmol/L), or PMSF (1 mmol/l). Loxosceles venom or toxins were added and incubated 30 minutes 37°C. Cells were washed and analyzed for C susceptibility (A), expression of GPC using mAb Bric4 (B), incorporation of toxins using Ab F35 (C). Results are mean ± SEM of experiments carried out in triplicate.