P2 incorporates into E and causes dose-dependent cleavage in glycophorin expression and hemolytic susceptibility.

(A) E were treated with various concentrations of P2 and analyzed by flow cytometry using the mAb for GPA (□), GPB (⋄), and GPC (▵) or the polyclonal serum F35 against P2 (○). Cells were also subjected to C in a hemolysis assay (•). (B) E ghosts obtained from samples described above and were prepared and analyzed by Western blot using the monoclonal antibody against the intracellular portion of GPA (Bric163).