Loxosceles venom and purified toxins do not cleave purified glycophorin A.

Purified GPA (2 μg) was incubated with buffer (C) or with 2 μg ofL. intermedia venom (V), P1, P2, or P3 toxins for 60 minutes at 37°C. Samples were separated by SDS-PAGE under nonreducing conditions (12% gel) and analyzed by Western blot using the monoclonal antibody Bric163 .