Fig. 3.
Cleavage fragments of GPA, GPB, and GPC remain on E ghosts after treatment with L. intermedia venom toxins.
E were treated with VBS2+ (C) or with 10 μg/mL of L. intermedia venom (V) or purified toxins P1, P2, or P3. E ghosts were prepared and run on 12% SDS-PAGE under nonreducing conditions (A to D) or were stained with silver (E). Western blots: A, GPA extracellular epitope; B, GPA intracellular epitope; C, GPB extracellular epitope; D, GPC intracellular epitope.
