CrkL enhances the association of Bcr-Abl and GCKR.

HEK 293T cells were transfected with constructs that directed the expression of Bcr-Abl and HA-GCKR in the presence or absence of CrkL (lanes 1, 2 versus lanes 3, 4). Bcr-Abl (lanes 2, 4) or control (lanes 1, 3) immunoprecipitates were analyzed by immunoblotting for HA-GCKR. Cell lysates from the same transfection were analyzed for Bcr-Abl and HA-GCKR by immunoblotting with a Bcr-Abl or an HA antibody. This experiment was preformed twice with similar results.