Fig. 4.
![Fig. 4. Inhibition of APN activity on CEM and U937 cells. / (A) Assay of APN activity of CEM or U937 cells incubated for 6 hours with different concentrations of arphamenine B (CEM(arpha), U937(arpha)) or actinonin (CEM(act), U937(act)); mean percentages ± SD (n = 2) of the APN activity of treated cells relative to control cells without inhibitor. (B) CEM or U937 cells were cultured overnight in the presence of [3H]dThd, with or without arphamenine B or actinonin at the indicated concentrations; each bar corresponds to the mean (+ SD) of 3 to 8 independent determinations; the significance of differences between percentages of incorporated cpm and 100% control values was assessed by the 1 sample t test: ***P < .001.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/2/10.1182_blood.v95.2.453/6/bloo00223004x.jpeg?Expires=1724269493&Signature=lijmBdiSscMVheKjndwREDYXBts~tQfQ8q1usBKsUAQaL2BCz~UO9MVMBmyLOHwzysUBUPlDjqpxFPbnipVEEyN9O3nSN82Fx63p05wbEvGisjKyBLTsNuIOi34FU19aXahhfXlJqRfo75E3ob73-p4oEZLQgXXjJ-ZxPm0O6F65ezSDUcXQmX4Tpl5060lOH~u52w2HHWZq3lxqkvUrVusUrcJFwlyJv~gu8PCVRYb4rQW~IqrwCGk3eQQBT2c0Cq8DcZVpKCZaHJS3qq2mUJxfUC3H8GRsU8olti7uyjbcLUjS60tQRo18L9a3P7jX7srfYwCA1btty8xCDwfxwQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of APN activity on CEM and U937 cells.
(A) Assay of APN activity of CEM or U937 cells incubated for 6 hours with different concentrations of arphamenine B (CEM(arpha), U937(arpha)) or actinonin (CEM(act), U937(act)); mean percentages ± SD (n = 2) of the APN activity of treated cells relative to control cells without inhibitor. (B) CEM or U937 cells were cultured overnight in the presence of [3H]dThd, with or without arphamenine B or actinonin at the indicated concentrations; each bar corresponds to the mean (+ SD) of 3 to 8 independent determinations; the significance of differences between percentages of incorporated cpm and 100% control values was assessed by the 1 sample t test: ***P < .001.
