Fig. 3.
![Fig. 3. Effect of the inhibition of APN activity on cultured cell growth. / (A) Sorted CD13hi and CD13lo cells were incubated overnight in the presence of 3[H]dThd, with or without 10−4 mol/L arphamenine B (Arpha.B) or actinonin; data are from 1 experiment of 2. (B) Bulk culture day 5 or day 7 cells were treated in the same manner as in A; data are presented as mean [3H]dThd incorporation ± SD (n = 6); statistical significance of differences: P < .001 for actinonin vs control and vs arphamenine B. (C) Culture day 7 cells were incubated overnight with CD13 mAb F23, WM15 or TÜK1, or with isotype control mAb IgG2a for F23 and IgG1 for WM15 and TÜK1 (all at 50 μg/mL) in the presence of [3H]dThd; data are from 1 experiment of 2.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/2/10.1182_blood.v95.2.453/6/bloo00223003x.jpeg?Expires=1724268244&Signature=EgInoMuCL-6qOXOXqfESfNS2e5BIGB6G-~LztcxyrxQSlIp3EemKhqX6Gr-EYOsn1HgXSsSz9YmrgVLwOh1B-vReUU-q25vQYLt2QZJh27ZX5rpXiY4RzH0QqEq87MNyUSLwjIjikqhur60N2iKTwupDjG1b1vmXGSs1PN7RXko~PfNhK5K5QoY5CVVvTHcKeVIYHoZULs7zSBv8ep26bcP0t9eblkCCPAaTXK3CAvezCBOjIpdfIRQz56cQN80zEN5uxgWIsY37Q6JVmOYhppEtRJo0ClXyes69vXsnuV75A2p217IVM3PpICcZFJx9jWUF9K1iiiBNNhX7mHIi0A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of the inhibition of APN activity on cultured cell growth.
(A) Sorted CD13hi and CD13lo cells were incubated overnight in the presence of 3[H]dThd, with or without 10−4 mol/L arphamenine B (Arpha.B) or actinonin; data are from 1 experiment of 2. (B) Bulk culture day 5 or day 7 cells were treated in the same manner as in A; data are presented as mean [3H]dThd incorporation ± SD (n = 6); statistical significance of differences: P < .001 for actinonin vs control and vs arphamenine B. (C) Culture day 7 cells were incubated overnight with CD13 mAb F23, WM15 or TÜK1, or with isotype control mAb IgG2a for F23 and IgG1 for WM15 and TÜK1 (all at 50 μg/mL) in the presence of [3H]dThd; data are from 1 experiment of 2.
