Effects of rhIL-11 on colony formation of murine bone marrow 2d 5FU Lin⁻Sca-1⁺c-kit⁺ cells.

Sorted bone marrow cells (100-200 cells) were plated in 0.8% methylcellulose containing aMEM medium and 30% FCS in a total volume of 1.5 mL. (left) Culture contained 100 ng/mL rmSF. (right) Culture was supplemented with purified cytokines at the following concentrations: 100 ng/mL rhIL-11 and rmSF and 20 ng/mL rmIL-3. Target cell population in each panel was Lin⁻Sca-1⁺c-kit⁺ cells from 2d 5FU-treated C57Bl/6 mice. Colony number and size were scored after 10 to 12 days of incubation.