cPLA₂ mRNA levels during sepsis and after recovery.

(A) A representative RT-PCR experiment assessing cPLA₂ mRNA levels of a septic patient manifesting elevated cPLA₂protein activity and expression. The amplified products of cPLA₂ (628 bp) and the corresponding products of β-actin (269 bp) are shown. The PCR reactions were amplified for 25, 35, and 45 cycles (A, B, and C, respectively). The first lane shows the 123 bp size marker. (B) cPLA₂ mRNA levels in human neutrophils during sepsis and after recovery. The levels of cPLA₂ mRNA in neutrophils of the septic patients with increased cPLA₂and the controls were quantitated by densitometry and divided by the corresponding actin mRNA levels. The mean ± SEM of the densitometry analysis in the septic patients and the controls are shown. The densitometric analysis demonstrates that cPLA₂ mRNA levels are significantly higher (P < 0.001) during the disease than after recovery.