American Society of Hematology

Fig. 3.

$Fig. 3. Colocalization of PI3K p55, p85, and p85β to lipid bodies of murine RAW cells. / Lipid body and other subcellular fractions were isolated from RAW cells as described in “Materials and Methods.” A positive control adipocyte lysate (100 μg) and proteins (20 μg) from each subcellular fraction of RAW cells were electrophoresed and immunoblotted with anti-PI3K p85 mAb (A), a PI3K p85α specific pAb (B), an anti-MAP kinase anti-pan ERK mAb (C), and an anti-protein 14-3-3β pAb (D).$

Colocalization of PI3K p55, p85, and p85β to lipid bodies of murine RAW cells.

Lipid body and other subcellular fractions were isolated from RAW cells as described in “Materials and Methods.” A positive control adipocyte lysate (100 μg) and proteins (20 μg) from each subcellular fraction of RAW cells were electrophoresed and immunoblotted with anti-PI3K p85 mAb (A), a PI3K p85α specific pAb (B), an anti-MAP kinase anti-pan ERK mAb (C), and an anti-protein 14-3-3β pAb (D).

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