Characterization of hFVIII secreted in mouse plasma.

Mouse plasma from a treated C57BL/6 mouse was used for immunoprecipitation of hFVIII and Western blot analysis as in Figure 2. (left) FVIII heavy chain. (right) FVIII light chain. (lane 1) Normal mouse plasma. (lane 2) Mouse plasma from a treated C57BL/6 mouse. (lane 3) Recombinant FVIII spiked in normal mouse plasma. (arrows) FVIII-specific signals. HC, heavy chain; LC, light chain.