Fig. 4.
Detection of humoral immune responses in hemophilic mice.
(A) Example of anti-hFVIII antibody responses in hemophilic mice 52, 62, and 68 as measured by ELISA. The mice received MiniAdFVIII by tail vein injection at 2 × 1011 vp/mouse. Representative individuals are shown. (B) Anti-Ad antibody responses in hemophilic mice 28, 46, 47, and 48. The mice were given a single injection of MiniAdFVIII (2 × 1011 vp/mouse) and bled at various time points after injection. The anti-Ad IgG titers were measured by ELISA. Endpoint titers are defined as the reciprocal of the last serum dilution giving a half-maximal OD490 reading of 1.5.
