Characterization of hFVIII produced in MiniAdFVIII-infected cells.

(A) Production of functional hFVIII secreted into the supernatant of infected 293 cells. (B) Conditioned medium from HepG2 infected cells was used for hFVIII immunoprecipitation with a specific antibody to the heavy chain. Immunodetection was performed on nitrocellulose membranes with specific antibodies to the heavy (left) or light chains (right). (lane 1) Conditioned medium from uninfected cells. (lane 2) Conditioned medium from infected cells. (lane 3) Recombinant FVIII spiked in conditioned medium from uninfected cells. (arrows) FVIII-specific signals. HC, heavy chain; LC, light chain.