Fig. 1.
Fig. 1. (A) Structure of MiniAdFVIII and ancillary vectors. / MiniAdFVIII is devoid of all viral genes. The presence of the adenovirus ITRs and packaging signal (Ψ) allows the MiniAdFVIII vector to replicate and package efficiently in cells coinfected with the ancillary vector and expressing adenovirus E1 early proteins. (arrow) Direction of transcription. The ancillary vector is an E1-substituted adenovirus with a partially deleted packaging signal. (B) Detection of ancillary vector in MiniAdFVIII preparations. Total viral DNA from 6 × 1010 viral particles was extracted and digested with PshAI. The expected bands for MiniAdFVIII are 17 300, 9949, 3233, and 627 bp; for the ancillary vector, they are 21 382, 8497, and 7770 bp, respectively. The position of the bands for MiniAdFVIII (9949 bp) and ancillary (8497 bp and 7770 bp) vectors are indicated. (1) Molecular weight markers, 1-kb ladder (GIBCO). (2) Viral DNA digested with PshAI restriction enzyme.

(A) Structure of MiniAdFVIII and ancillary vectors.

MiniAdFVIII is devoid of all viral genes. The presence of the adenovirus ITRs and packaging signal (Ψ) allows the MiniAdFVIII vector to replicate and package efficiently in cells coinfected with the ancillary vector and expressing adenovirus E1 early proteins. (arrow) Direction of transcription. The ancillary vector is an E1-substituted adenovirus with a partially deleted packaging signal. (B) Detection of ancillary vector in MiniAdFVIII preparations. Total viral DNA from 6 × 1010 viral particles was extracted and digested with PshAI. The expected bands for MiniAdFVIII are 17 300, 9949, 3233, and 627 bp; for the ancillary vector, they are 21 382, 8497, and 7770 bp, respectively. The position of the bands for MiniAdFVIII (9949 bp) and ancillary (8497 bp and 7770 bp) vectors are indicated. (1) Molecular weight markers, 1-kb ladder (GIBCO). (2) Viral DNA digested with PshAI restriction enzyme.

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