The effect of the RAR antagonist, AGN 193 109, on liquid suspension cultures of short- and long-term repopulating hematopoietic stem cells.
2000 FACS-enriched hematopoietic precursors (lin− c-kit+ Sca-1+) were added to wells containing media and cytokines (SCF, IL-6, IL-11, and Flt-3-ligand), and cultured without (▪) or with (○) 1 μmol AGN 193109. Irradiated Ly5.1 recipients (5 mice per group) were transplanted with 1 × 105 normal Ly5.1 bone marrow cells together with all cells that grew from 1000 lin− c-kit+ Sca-1+ precursors after 7 days in liquid suspension culture. Data are expressed as the mean ± SEM donor cell reconstitution in the peripheral blood of transplanted recipients analyzed between 5 weeks (1.25 mo) and 6 months after transplant. Results of 7-day cultured cells (A, B) are shown from 2 separate experiments.
