American Society of Hematology

Fig. 5.

$Fig. 5. Southern blot analysis. / (A) Circulating leukocytes from animal 94E041 were collected from whole blood at 6 weeks and fractionated into mononuclear (MN) and granulocyte (Gran) fractions using a density centrifugation as previously described. Similarly bone marrow MN cells were also isolated at the same time point. Quantitative Southern blot analysis was performed on the isolated DNA, and the calculated copy number is shown and compared to the proportion of transduced cells as determined by flow cytometric analysis. The flow data are shown for EGFP-expressing lymphocytes (L), monocytes (M), and granulocytes (Gran). (B) Southern blot analysis of DNA obtained from PBMN cells in the 3 high-dose animals. The sample was obtained at the indicated number of weeks after transplant. Also shown is the single copy fibroblast control (Pos.) and DNA from an untransplanted animal (Neg.).$

Southern blot analysis.

(A) Circulating leukocytes from animal 94E041 were collected from whole blood at 6 weeks and fractionated into mononuclear (MN) and granulocyte (Gran) fractions using a density centrifugation as previously described. Similarly bone marrow MN cells were also isolated at the same time point. Quantitative Southern blot analysis was performed on the isolated DNA, and the calculated copy number is shown and compared to the proportion of transduced cells as determined by flow cytometric analysis. The flow data are shown for EGFP-expressing lymphocytes (L), monocytes (M), and granulocytes (Gran). (B) Southern blot analysis of DNA obtained from PBMN cells in the 3 high-dose animals. The sample was obtained at the indicated number of weeks after transplant. Also shown is the single copy fibroblast control (Pos.) and DNA from an untransplanted animal (Neg.).

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