Time course evaluation of EGFP expression in peripheral blood cells.

In the upper panels, the percentage of circulating lymphocytes (•), monocytes (▾), and granulocytes (○) expressing EGFP was evaluated over a course of 35 weeks in animals RC601, 95E041, and RC502 receiving cells transduced with MGirL22Y-Pre vector containing, on treated 6-well plates, media supplemented with SCF, 300 ng/mL; flt-3, 300 ng/mL; and IL-6, 50 ng/mL. The lower panels show comparative analysis of EGFP expression in platelets (□) and red blood cells (▪) over time.