EGFP expression in circulating leukocyte populations.

Flow cytometric analysis was performed on the circulating leukocytes of a transplanted animal (95E041) at 2 weeks (A) and 10 weeks (B) following transduction and reinfusion of autologous immunoselected CD34⁺ cells. The immunoselected CD34⁺ cells were transduced using treated nontissue culture plates and maintained in MGirL22Y-Pre vector containing media supplemented with 300 ng/mL SCF, 300 ng/mL flt-3, and 50 ng/mL IL-6. The circulating leukocyte populations were gated, as shown, into lymphocyte, monocyte, and granulocyte populations based on their forward and 90°C light scatter properties and evaluated for EGFP expression.