Representative fluorescence-activated cell sorter (FACS) dot plots of granulocyte-derived microparticles in plasma from a patient who survived meningococcal sepsis.

Microparticles were isolated from the plasma of a (surviving) patient on admission, labeled, and analyzed by flow cytometry as described in “Materials and Methods.” (A) Unlabeled microparticles (autofluorescence); (B) labeled with annexin V–PE; (C) labeled with IgG₁-FITC (control mAb); (D) labeled with anti-CD66b–FITC; and (E) double staining with annexin V–PE and anti-CD66b–FITC. (F) Representative dot plot of microparticles isolated from healthy volunteer plasma, double stained with annexin V–PE and anti-CD66b–FITC, for comparison.