Fig. 5.
Infection of mature megakaryocytes with HCMV.
Cultured cells were purified from thrombopoietin-supplemented cultures of CD34+ cells using magnetic beads based on the expression of CD42a antigen after 12 days of culture. Cells were counter-stained with DAPI and photographed at ×600 magnification. (A) HCMV-infected cells. CD42a-FITC versus biotinylated HCMV anti-IE and streptavidin PE. (B) HCMV-infected cells: murine HCMV anti-pp65 IgG2 mAb and antimouse IgG-PE. (C) HCMV-infected cells: CD42a-FITC versus biotinylated HCMV anti-IE and streptavidin PE. (D) HCMV-infected cells: isotype controls.
