Flow cytometric analysis of magnetic bead-purified CD42a⁺ cells derived from CD34⁺ cell cultures supplemented with thrombopoietin.

Cultured cells were harvested from TPO-supplemented cultures of CD34⁺ cells using magnetic beads based on peak expression of CD42a antigen after 12 days of culture. Gates were set based on the isotypic controls. Log PE fluorescence activity (CD34) versus log fluorescein isothiocyanate activity (CD42a). (A, C) Cells before CD42a-enrichment step. (B, D) Cells after enrichment step. (A, B) Cells derived from uninfected cultures. (C, D) Cells derived from infected cultures.