Western blot analysis of neutrophil membranes reisolated from the cell-free activation system.

The reisolated membranes were prepared as described in the legend of Table 2. Four fifths of these membrane samples were precipitated with 10% (w/v) trichloric acid and resuspended in sample buffer as previously described. After 10% PAGE, the proteins were blotted and stained for p47-phox and p67-phox.